Debottlenecking the strictosidine pathway - and improving workflows with teemi
By Søren Petersen
9 October 2024
Pathway
Cytochrome P450s (CYPS)
Strategies for debottlenecking
Main hypothesis
Specific combinations of CPR / G8H homologs and corresponding expression levels can remove the G8H bottleneck in the Strictosidine pathway
Experimental setup:
Results
Results
Teemi
Project parts
Design
Promoter & homolog selection
Build
Background strain
Library
Test
Library characterization
Learn
Plotting and modelling
Project notebooks
Project notebooks
1. Organise samples
2. Simulate experiments
3. Plan reactions
Summary
Benefits of literate programming (as I see them)
One system
Automate everything repetitive
Error reduction
Next level documentation
Resist knowledge loss
Ultimate goal: more time for biological questions
Thank you for your attention!